Senior PD Upstream Scientist RAAV Vector Engineering

Full-Time
Philadelphia, PA
Spark Therapeutics
Posted 2 years ago – Accepting applications

Job Description

Join the Spark Team

We were born of innovation, springing from the curiosity, imagination and dedication of remarkable scientists and healthcare visionaries. Our shared mission is to challenge the inevitability of genetic disease by discovering, developing, and delivering treatments in ways unimaginable – until now.

We don’t follow footsteps. We create the path.

The rAAV Vector Engineering Sr. Scientist is involved in the execution of recombinant adeno-associated viral vector (rAAV) production processes. The Sr. Scientist will participate in research to develop AAV vectors, vector engineering, Proof of concept (POC) studies, execute experiments, develop protocols and relevant supporting documentation. The Sr. Scientist position requires experience with molecular biology, biochemistry and gene expression analyses. He/she will be responsible for:

Conducting gene editing vector design, molecular cloning, plasmid prep, and sequencing
Conducting AAV vector engineering and production
Conducting various assays to validate and screen plasmid constructs
Screening of genetic elements and design of next generation rAAV molecular expression systems
Contribute to experimental design, data analysis, and report
Maintain detailed and well-organized documentation with best industry practice
Self-motivated and a passion for working in gene therapy
Implementing plasmid engineering strategies to develop improved AAV production for gene therapy purposes.
Experience with design, execution and data analysis related to experimental studies utilizing in vitro models.
Collaborating with other Process Development Scientists at Spark to design and conduct in vitro studies to optimize development of expression vectors
Participating in joint programs and collaborations with external parties.
Writing study reports, manuscripts, technical reports and present results at appropriate scientific meetings.
Preparing and reviewing process development protocols, data analysis summaries, technical reports, and other relevant process documentation.
Maintaining an accurate laboratory notebook.
Ensuring work conforms to applicable quality and safety standards.
Successful training of colleagues on various experimental assays
Participating on project teams (as needed).

Familiarity with gene therapy and/or AAV is preferred but not required.

Responsibilities

Perform laboratory support work and execute laboratory experiments.

Analyze data from experiments and draft presentations.

Draft protocols for studies and the subsequent technical reports as needed.

Attend meetings and perform administrative duties as needed.

Education and Experience Requirements

Ph.D. in Biology, Molecular Biology, Microbiology, Virology, Biochemistry, Chemistry, Chemical Engineering or related discipline with 3-5 years of relevant experience.
B.S./M.S. in Biology, Virology, Cell Biology, Molecular Biology, Biochemistry, Chemical Engineering or a related discipline with 6-8 years of relevant experience
Extensive hands-on experience in gene and vector editing, and transcriptional and translational analysis.
Proficient in sub-cloning, transfection, Western, ELISA, PCR, and qPCR techniques
Experience with viral vector production is preferred but not required.

Key Skills, Abilities, and Competencies

Prior experience with gene editing technologies (e.g., CRISPR/Cas9, TALEN, ZFN, etc)
Experience in culturing mammalian cell lines and transfection.
Experienced in advanced lab techniques such as plasmid design and construction, molecular cloning, protein expression, qPCR, cell culture, ELISA, plate-based luciferase and/or enzymatic activity assays, SDS-PAGE/western blotting, gene expression assays, etc.
Knowledge of Next Generation Sequencing techniques is preferred
General knowledge of regulatory requirements for GMP manufacturing of biologics or gene therapy vectors are preferred, but not required
Familiarity with designing genetic control elements using a combination of molecular, cellular and in vitro techniques.
Strong analytical skills and problem-solving capabilities.
Proven ability to work independently in hands-on laboratory setting, self-motivated to learn and develop new techniques, perform multiple tasks simultaneously, keep accurate records, follow instructions, and comply with company policies.
Working knowledge of state-of-the-art principles, technologies, and instrumentation.
Excellent communication skills (verbal, written, interpersonal). Must be able to draft protocols and technical reports, and provide effective technical presentations.
Ability to work collaboratively across a development environment with cross-functional teams.
Maintain relevant laboratory supplies and equipment as needed
Adaptability to emerging project/priority needs.
Analytical and problem-solving skills.
Ability to manage time effectively.
Ability to communicate and negotiate with vendors in a strategic manner.
Ability to work well under pressure and meet deadlines.
Proficiency in MS Office, JMP, Phyton and R/RStudio is a plus.

Complexity and Problem Solving

The Molecular AAV Engineering Sr. Scientist is expected to use scientific principles and practices to solve a range of problems in creative and practical ways. The Molecular AAV Engineering Sr. Scientist is expected to work with the Cell line Development and Molecular AAV Engineering Team in executing experiments, interpreting results, and performing any necessary troubleshooting.

Internal and External Contacts

Fellow Upstream PD Team Members (Cell Line and Cell Culture Development Teams)
Cross-functional Team Members such as: PD Downstream, PD Analytical, Automation, PCO, Facilities, and Service Personnel/Vendors.
Discovery Team
External Vendors that offer equipment and/or services for various aspects of Process Development.

Other Job Requirements

Must be able to lift 25 pounds.
Due to the inherent nature of cell culture, some weekend work and/or work on holidays may be needed. On occasion, long work hours may be required depending upon the experiment schedule.
Handling large volumes of cell culture or related materials, such as tens of liters, may be required. Also, several viral materials, including AAV, adenovirus and lentivirus may be involved in the work.

Conducting gene editing vector design, molecular cloning, plasmid prep, and sequencing
Conducting AAV vector engineering and production
Conducting various assays to validate and screen plasmid constructs
Screening of genetic elements and design of next generation rAAV molecular expression systems
Contribute to experimental design, data analysis, and report
Maintain detailed and well-organized documentation with best industry practice
Self-motivated and a passion for working in gene therapy
Implementing plasmid engineering strategies to develop improved AAV production for gene therapy purposes.
Experience with design, execution and data analysis related to experimental studies utilizing in vitro models.
Collaborating with other Process Development Scientists at Spark to design and conduct in vitro studies to optimize development of expression vectors
Participating in joint programs and collaborations with external parties.
Writing study reports, manuscripts, technical reports and present results at appropriate scientific meetings.
Preparing and reviewing process development protocols, data analysis summaries, technical reports, and other relevant process documentation.
Maintaining an accurate laboratory notebook.
Ensuring work conforms to applicable quality and safety standards.
Successful training of colleagues on various experimental assays
Participating on project teams (as needed).

Familiarity with gene therapy and/or AAV is preferred but not required.

Responsibilities

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Job Function and Description

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