Research Technician II Details

Research Technician II

Baylor College of Medicine - Houston, TX

Employment Type : Full-Time

Summary

The Dere Lab in the Center for Precision Environmental Health is looking for a talented individual to join the team. The Dere lab focuses on studying a novel role for chromatin modifiers, often lost in renal cell carcinoma, in regulating cytoskeletal dynamics in cellular processes such as mitosis and establishment of primary cilia during quiescence. The laboratory utilizes several unique methodologies including biochemistry, genetics, mass spectrometry, imaging and other cell biology-based assays. This position requires a technician who is self-motivated and would principally be involved in performing experiments outlined by the Principal investigator. Research in the lab would include cell culture, maintaining human and mouse cell lines, transfections and infections of cells in culture, generating stable cell lines expressing gene of interest. Additionally, the technician would entail biochemical experiments that will include protein expression, purifications, methylation/demethylation and kinase assays, western analysis, immunoprecipitations, and fluorescent staining of cells. The technician will also isolate DNA, maintain buffer stocks and other laboratory stocks.

• Prepares and conducts laboratory research experiments following SOPs.
• Collects, compiles, evaluates and analyzes data.
• Interprets detailed assay procedures and test results.
• Prepares reagent, solutions, and oligos used in the lab.
• Troubleshoots problematic results and consults with senior lab members on improving results.
• Keeps a detailed laboratory manual.
• Performs research that includes cell culture, maintaining human and mouse cell lines, transfections and infections of cells in culture, generating stable cell lines expressing gene of interest.
• Conducts biochemical experiments that will include protein expression, purifications, methylation/demethylation and kinase assays, western analysis, immunoprecipitations, and fluorescent staining of cells.
• Works on isolation of DNA, maintains buffer stocks and other laboratory stocks.

• Bachelor's degree in a Basic Science or related field. Four years of related experience may substitute for degree requirement.
• No experience required.

• Bachelor's degree in biological science or equivalent.
• Molecular Biology skills including nucleic acid handling, enzymatic reaction, PCR reaction, etc.
• Experience in basic protocols in molecular biology and a quick learner.
• Flexible and adaptive to the lab environment. Committed to finish the goals in a timely manner.
• Good at multi-tasking, problem-solving, and have strong communication skills.

Baylor College of Medicine requires employees to be fully vaccinated -subject to approved exemptions-against vaccine-preventable diseases including, but not limited to, COVID-19 and influenza.

Baylor College of Medicine is an Equal Opportunity/Affirmative Action/Equal Access Employer.

10587

CA